The Food Safety and Inspection Service (FSIS) is announcing changes in procedures for certain Listeria (L.) monocytogenes sampling programs in ready-to-eat (RTE) meat and poultry products. Starting 60 days after issuance of this notice, FSIS will increase the number of product samples its laboratories analyze under its Routine Risk-Based L. monocytogenes (RLm) Sampling Program and its Intensified Verification Testing (IVT) protocol. Currently, a sampling unit for both of these sampling programs consists of 10 food-contact surface, five non-food-contact surface samples, and three food product samples. FSIS laboratories will increase the number of product samples from three to five samples per sampling unit. In addition, FSIS laboratories will composite the five 25 g samples from the RLm sampling program, which will increase the sample size of the analyzed test portion from 25 g to 125 g. The Agency is initiating these changes to make its sampling procedures more consistent with international practices, to conserve its laboratory resources, and to improve public health. FSIS invites comments on these changes to its sampling programs.